منابع مشابه
Slow cruciform transitions in palindromic DNA.
Extrusion of cruciform structures in self-complementary regions of DNA is known to be favored by negative supercoiling of DNA. We show here that, in moderately supercoiled DNA, cruciform extrusion is a very slow process. In plasmid pUC7 DNA, with a 48-base-pair palindrome, the half-time of extrusion at 50 degrees C is typically several hours; rates are even slower at lower temperature. The rate...
متن کاملComparison of physical and genetic properties of palindromic DNA sequences.
Some viable palindromic DNA sequences were found to cause an increase in the recovery of genetic recombinants. Although these palindromes contained no Chi sites, their presence in cis caused apparent recA+-dependent recombination to increase severalfold. This biological property did not correlate with the physical properties of the palindromes' extrusion of cruciform structures in vitro. Thus, ...
متن کاملStructural intermediates of deletion mutagenesis: a role for palindromic DNA.
A model is presented for deletion mutations whose formation is mediated by palindromic and quasipalindromic DNA sequences. It proposes that the self-complementarity of palindromes allows the formation of DNA secondary structures that serve as deletion intermediates. The structures juxtapose the end points of the deletion and thus direct deletion specificity. While misaligned DNA intermediates t...
متن کاملUnusual aggregation behavior of colloids coated with palindromic DNA
Submitted for the MAR08 Meeting of The American Physical Society Unusual aggregation behavior of colloids coated with palindromic DNA MIRJAM LEUNISSEN, REMI DREYFUS, DAVID PINE, PAUL CHAIKIN, New York University — Coating particles with single-stranded DNA ‘sticky ends’ gives excellent control over the specificity, strength and range of their interactions. Usually, a pair of complementary ‘Wats...
متن کاملPalindromic repetitive DNA elements with coding potential in Methanocaldococcus jannaschii.
We have identified 141 novel palindromic repetitive elements in the genome of euryarchaeon Methanocaldococcus jannaschii. The total length of these elements is 14.3kb, which corresponds to 0.9% of the total genomic sequence and 6.3% of all extragenic regions. The elements can be divided into three groups (MJRE1-3) based on the sequence similarity. The low sequence identity within each of the gr...
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ژورنال
عنوان ژورنال: The Journal of General and Applied Microbiology
سال: 1986
ISSN: 1349-8037,0022-1260
DOI: 10.2323/jgam.32.13